Journal: Animal Nutrition

Article Title: Delivery routes for probiotics: Effects on broiler performance, intestinal morphology and gut microflora

doi: 10.1016/j.aninu.2015.07.002

Figure Lengend Snippet: Bacterial counts (lg cfu/g) in the digesta of birds on d 7 and 21. 1

Article Snippet: Lactobacilli were enumerated on Rogosa agar (Oxoid, CM 0627) after anaerobic incubation at 39°C for 48 h. Numbers of Clostridium perfringens ( Cp ) were counted on Tryptose–Sulfite–Cycloserine and Shahidi-Ferguson Perfringens agar base (TSC & SFP) (Oxoid, CM0587 OPSP) mixed with egg yolk emulsion (Oxoid, SR0047) and Perfringens (TSC) selective supplement (Oxoid, SR0088E) according to the pour-plate technique, where plates were overlaid with the same agar after spreading the inoculums and incubated anaerobically at 39°C for 24 h. All plates were incubated in the anaerobic cabinet (Model SJ-3, Kalter Pty.

Techniques:

Journal: Food Science and Biotechnology

Article Title: Evaluation of the microbial contamination of fresh produces and their cultivation environments from farms in Korea

doi: 10.1007/s10068-019-00570-3

Figure Lengend Snippet: Culture media a and identification methods for pathogenic bacteria isolated from fresh produce in this study

Article Snippet: Rappaport–Vassiliadis R10 broth (Difco) Tetrathionate broth (Difco) Xylose lysine desoxycholate agar (XLD, Difco) API 20E (BioMeriux) – – Staphylococcus aureus Tryptic soy broth (Difco) in 10% NaCl Baird-Parker agar (BPA, Difco) API Staphy (BioMeriux) – (SolGent co.,Ltd., Korea) Escherichia coli O157:H7 E.C. broth (Oxoid, Hampshire, UK) Sorbitol MacConkey agar (SMAC, Difco) – E. coli O157 Diagnostic Reagents test kit (Oxoid) – Clostridium perfringens Cooked meat (Difco) Perfringens agar base (TSC, Difco) – – – Open in a separate window a Culture media for pathogens quoted from the MFDS Food Code b The only three samples showing positive results on culture media methods for E. coli O157:H7 or L. monocytogenes were identified using Latex agglutination test method c Samples showing positive results with culture media methods were subsequently subjected to the 16s rRNA method d Not tested Culture media a and identification methods for pathogenic bacteria isolated from fresh produce in this study

Techniques: Isolation, Agglutination, Sequencing, Diagnostic Assay

Journal: Food Science and Biotechnology

Article Title: Evaluation of the microbial contamination of fresh produces and their cultivation environments from farms in Korea

doi: 10.1007/s10068-019-00570-3

Figure Lengend Snippet: Culture media a and identification methods for pathogenic bacteria isolated from fresh produce in this study

Article Snippet: The enriched broth was streaked on a Perfringens agar base (TSC, Difco).

Techniques: Bacteria, Isolation, Agglutination, Sequencing, Diagnostic Assay

Journal: Food Science and Biotechnology

Article Title: Evaluation of the microbial contamination of fresh produces and their cultivation environments from farms in Korea

doi: 10.1007/s10068-019-00570-3

Figure Lengend Snippet: Numbers of positive samples with pathogenic contamination of fresh produce

Article Snippet: The enriched broth was streaked on a Perfringens agar base (TSC, Difco).

Techniques:

Journal: Veterinary World

Article Title: Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

doi: 10.14202/vetworld.2021.2809-2816

Figure Lengend Snippet: Prevalence of C. perfringens in broiler farms of Mymensingh district of Bangladesh.

Article Snippet: A loopful of positive culture was streaked on perfringens agar base (TSC; HiMedia, India) with selective supplement (HiMedia) and incubated in the anaerobic jar at 37°C for 24 h. Successively, cultured plates were observed for the growth of C. perfringens and colony morphology was evaluated and recorded concurrently.

Techniques: Environmental Sampling

Journal: Veterinary World

Article Title: Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

doi: 10.14202/vetworld.2021.2809-2816

Figure Lengend Snippet: Agarose gel electrophoresis showing 402 bp amplicon of alpha-toxin gene ( cpa gene) of Clostridium perfringens . Lane M: 100 bp DNA marker; lane N: Negative control; lanes 1-4: Isolates positive for C. perfringens .

Article Snippet: A loopful of positive culture was streaked on perfringens agar base (TSC; HiMedia, India) with selective supplement (HiMedia) and incubated in the anaerobic jar at 37°C for 24 h. Successively, cultured plates were observed for the growth of C. perfringens and colony morphology was evaluated and recorded concurrently.

Techniques: Agarose Gel Electrophoresis, Amplification, Marker, Negative Control

Journal: Veterinary World

Article Title: Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

doi: 10.14202/vetworld.2021.2809-2816

Figure Lengend Snippet: Result from univariable logistic regression analysis displaying the relationship with farm level C. perfringens infection in 40 broiler farms.

Article Snippet: A loopful of positive culture was streaked on perfringens agar base (TSC; HiMedia, India) with selective supplement (HiMedia) and incubated in the anaerobic jar at 37°C for 24 h. Successively, cultured plates were observed for the growth of C. perfringens and colony morphology was evaluated and recorded concurrently.

Techniques: Infection

Journal: Veterinary World

Article Title: Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

doi: 10.14202/vetworld.2021.2809-2816

Figure Lengend Snippet: Factors retained in the final multivariable mixed-effect logistic regression model of risk of flock level C. perfringens infection.

Article Snippet: A loopful of positive culture was streaked on perfringens agar base (TSC; HiMedia, India) with selective supplement (HiMedia) and incubated in the anaerobic jar at 37°C for 24 h. Successively, cultured plates were observed for the growth of C. perfringens and colony morphology was evaluated and recorded concurrently.

Techniques: Infection

Journal: Veterinary World

Article Title: Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

doi: 10.14202/vetworld.2021.2809-2816

Figure Lengend Snippet: Antimicrobial susceptibility status of C. perfringens isolates from 40 broiler flocks.

Article Snippet: A loopful of positive culture was streaked on perfringens agar base (TSC; HiMedia, India) with selective supplement (HiMedia) and incubated in the anaerobic jar at 37°C for 24 h. Successively, cultured plates were observed for the growth of C. perfringens and colony morphology was evaluated and recorded concurrently.

Techniques:

Journal: Veterinary World

Article Title: Investigation of Clostridium perfringens in small-scale commercial broiler flocks in Mymensingh district of Bangladesh

doi: 10.14202/vetworld.2021.2809-2816

Figure Lengend Snippet: Distribution of antimicrobial resistance status for Clostridium perfringens (n=41) strains isolated from broiler farms, R=Number of antibiotic resistances; AMX=Amoxicillin, CHL=Chloramphenicol, ERY=Erythromycin, OTE=Oxytetracycline, GEN=Gentamicin, n=Number of isolates with the indicated pattern.

Article Snippet: A loopful of positive culture was streaked on perfringens agar base (TSC; HiMedia, India) with selective supplement (HiMedia) and incubated in the anaerobic jar at 37°C for 24 h. Successively, cultured plates were observed for the growth of C. perfringens and colony morphology was evaluated and recorded concurrently.

Techniques: Isolation

Journal: Frontiers in Microbiology

Article Title: Probiotics Bacillus licheniformis Improves Intestinal Health of Subclinical Necrotic Enteritis-Challenged Broilers

doi: 10.3389/fmicb.2021.623739

Figure Lengend Snippet: Effects of B. licheniformis and enramycin the amounts of bacteria (lg CFU/g 1 ) in cecal digesta of broilers challenged with SNE.

Article Snippet: Commercial media were used for cultivation of C. perfringens (tryptose–sulfite–cycloserine agar, TSC, CM 138; Beijing Land Bridge Technology Co., Ltd.), coliform bacteria (Eosin–Methylene Blue Agar, EMB, CM105; Beijing Land Bridge Technology Co., Ltd.) and lactic acid bacteria (de Man, Rogosa, and Sharpe agar, MRS, CM 188; Land Bridge Technology Co., Ltd.).

Techniques: Bacteria

Journal: Animal Nutrition

Article Title: An early feeding regime and a high-density amino acid diet on growth performance of broilers under subclinical necrotic enteritis challenge

doi: 10.1016/j.aninu.2017.01.002

Figure Lengend Snippet: Interaction of necrotic enteritis challenge, feeding regime and diet on lesion score in 16-day-old broilers in the duodenum, jejunum and ileum. HAA = High amino acid starter diet; HELD = access to feed after 48 h post-hatch; FED = access to feed with 6 h post-hatch; Challenge = necrotic enteritis induced by oral administrations of Eimeria species and a field strain of Clostridium perfringens .

Article Snippet: The C. perfringens count in the inoculant had been quantified on perfringens tryptose-sulfite-cy-closerine (TSC) selective agar (Oxoid) following serial dilutions.

Techniques: